The possibility to perform single cell analysis, the speed of analysis (> 10,000 cells / sec) and the opportunity to analyze up to 20 parameters simultaneously (polychromatic analysis) make flow cytometry a powerful technique to quickly characterize biological multiples profiles (proteins, epitopes, nucleic acids, ion concentration, high content) in a single cell, in a very high number of cells (high throughput). “Deep profiling” of single cell through the diversity of cellular subtypes is complementary to “omics” technologies and is particularly important to understand the complexity of cell biological functions, such as intracellular signal, and mechanisms of differentiation.
- Becton Dickinson LSRFortessa X-20. Benchtop flow cytometer equipped with three lasers (488nm-blue, 640nm-red and 405 nm), with the possibility of being equipped with 5 lasers, for the simultaneous acquisition of 14 fluorescence parameters, in addition to 2 physical parameters. Equipped with automatic acquisition system for reading 96- 384-well plates for high-throughput analysis.
- Becton Dickinson FACSAria Fusion. A cell sorter that delivers high multicolor performance, equipped with five lasers (488nm, 561nm, 640nm, 405 nm e 355 nm), allows the simultaneous analysis of 18 parameters (16 fluorescence and 2 scatter). It is used to separate up to 4 different cell populations simultaneously, based on multidimensional phenotypic and / or functional analyses. Sorted cell subsets (up to 100% purity) are viable and can be grown and / or used for subsequent genomic-functional analyses.
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Platform Manager: Giulia Finotti (045 8027212)